First report of BVDV antigen from pneumonic cattle in Sudan

Intisar Kamil Saeed1,*,#, Yahia Hassan Ali1,#, Khalid Mohammed Taha2, Nada ElAmin Mohammed3, Yasir Mehdi Nouri4, Baraa Ahmed Mohammed1, Osama Ishag Mohammed5, Salma Bushra Elmagbool6 and Fahad Elghazali6
(PDF) Bovine viral diarrhea virus infection in cattle suffering from pneumonia in Sudan. Available from: https://www.researchgate.net/publication/272370293_Bovine_viral_diarrhea_virus_infection_in_cattle_suffering_from_pneumonia_in_Sudan.

  • 1Virology Department, Veterinary Research Institute, PO Box 8067, Al Amarat, Khartoum, Sudan;

    2Atbara Veterinary Research Laboratory, PO Box 121 Atbara, River Nile State, Sudan;

    3Wad Medani Veterinary Research Laboratory, PO Box 555, Gezira State, Sudan;

    4ElObied Veterinary Research Laboratory, PO Box 373, North Kordofan State, Sudan;

    5Rabak Veterinary Research Laboratory, PO Box 293 White Nile state, Sudan;

    6Molecular Biology Department, Veterinary Research Institute, PO Box 8067, Al Amarat, Khartoum, Sudan. #Current address: Northern Border University, Faculty of Science and Arts, Rafha, Saudi Arabia.

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To explore the expected role of Bovine Viral Diarrhea Virus (BVDV) in pneumonia in cattle, cattle lungs (n=242) showing signs of pneumonia were collected from slaughter houses of three different localities located at Northern, Central and Western Sudan during 2010–2013. The collected samples were tested for the presence of BVDV antigen using Enzyme-Linked Immunosorbent Assay (ELISA), and Fluorescent Antibody Test (FAT). Twenty six (10.7%) out of 242 samples were found to be positive for BVDV. Positive results were seen in all the three studied areas, with the highest prevalence (16.7%; n=4/24) at Gezira State in Central Sudan. BVDV genome could be detected in all ELISA positive samples. The results indicated the existence of BVDV infection in cattle in different areas in Sudan, and its possible association with respiratory infections in cattle. Analysis using BLAST indicated that the sequence was identical to the previously reported BVDV-1 (GenBank accession AF220247.1.); nucleotide A was found in our study at position 9 of our sequence, whereas T was present instead in the reference virus. This is the first report of detecting BVDV antigen, genome, and its sequence analysis collected from cattle lungs in Sudan.